Titlebook: Epigenome Editing; Methods and Protocol Albert Jeltsch,Marianne G. Rots Book 2024Latest edition The Editor(s) (if applicable) and The Autho

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https://doi.org/10.1007/978-3-322-82499-8of regulatory dependencies of the investigated chromatin regulator on endogenous cofactors. The system allows for an easy and dynamic readout at the single-cell level and the ability to compensate for cell-to-cell variances of transcription. The modular design of the system enables the simple adjust

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https://doi.org/10.1007/978-3-662-68494-8y optimization in cultured mammalian cells. Our protocol provides a comprehensive overview of flow cytometry analysis following transfection not just to improve transfection efficiency but also to assess the expression level of the utilized construct. We showcase our transfection protocol optimizati

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https://doi.org/10.1007/978-3-658-15658-9er. Major obstacles include the large size of dCas9 fusion proteins, the necessity of multimodal delivery of protein and gRNAs, and the potential of these formulations to elicit detrimental immune responses..In this context, viral methods for delivering CRISPR face several limitations, such as the p