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Titlebook: Extracellular Vesicles; Methods and Protocol Winston Patrick Kuo,Shidong Jia Book 2017 Springer Science+Business Media, LLC, part of Spring

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發(fā)表于 2025-3-21 17:07:09 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Extracellular Vesicles
副標(biāo)題Methods and Protocol
編輯Winston Patrick Kuo,Shidong Jia
視頻videohttp://file.papertrans.cn/320/319943/319943.mp4
概述Includes cutting-edge techniques for the study of extracellular vesicles.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Extracellular Vesicles; Methods and Protocol Winston Patrick Kuo,Shidong Jia Book 2017 Springer Science+Business Media, LLC, part of Spring
描述This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Comprehensive and practical, .Extracellular Vesicles: Methods and Protocols. serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications..
出版日期Book 2017
關(guān)鍵詞Biogenesis; Exosomes; Microvesicles; Inter-cellular communication; EVs; Isolation; Precision medicine
版次1
doihttps://doi.org/10.1007/978-1-4939-7253-1
isbn_softcover978-1-4939-8431-2
isbn_ebook978-1-4939-7253-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2017
The information of publication is updating

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發(fā)表于 2025-3-21 21:00:06 | 只看該作者
Purification Protocols for Extracellular Vesicless use antibody-coated beads to selectively isolate vesicles displaying a surface marker of interest. Ultimately, the choice of purification method for an individual experiment is influenced by time, cost, and equipment considerations, as well as the sample requirements for any downstream analyses.
板凳
發(fā)表于 2025-3-22 03:47:20 | 只看該作者
地板
發(fā)表于 2025-3-22 07:51:54 | 只看該作者
Christl A. Ruetzler,John M. Hallenbeck of a microenvironmental niche that promotes cancer cell survival, as well as reprogramming distant tissue for invasion. It is important to understand the mechanistic and functional aspects at the basic science level of EVs to get a better grasp on their role in healthy and disease states. EVs range
5#
發(fā)表于 2025-3-22 11:09:46 | 只看該作者
Rajeev K. Salunke,Murali Dharan,John W. Birks use antibody-coated beads to selectively isolate vesicles displaying a surface marker of interest. Ultimately, the choice of purification method for an individual experiment is influenced by time, cost, and equipment considerations, as well as the sample requirements for any downstream analyses.
6#
發(fā)表于 2025-3-22 12:58:59 | 只看該作者
1064-3745 tfalls.?.Comprehensive and practical, .Extracellular Vesicles: Methods and Protocols. serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications..978-1-4939-8431-2978-1-4939-7253-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
7#
發(fā)表于 2025-3-22 21:08:17 | 只看該作者
Oxidative Stress and Alzheimer’s Diseasen of exosome-sized extracellular vesicles. The approach is scalable and employs relatively gentle manipulation forces to fractionate and concentrate extracellular vesicles with good purity and functional integrity.
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發(fā)表于 2025-3-22 23:08:53 | 只看該作者
Steven D. Billings MD,Jenny Cotton MD, PhDstaining protocol for larger EVs isolated from human serum samples. We describe in detail the needed steps as currently done in our laboratory. Staining is demonstrated exemplarily for multi-antibody mix including CD147, a potential cancer marker if applied in combination with other MP/MV surface markers.
9#
發(fā)表于 2025-3-23 04:24:11 | 只看該作者
Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesiclesn of exosome-sized extracellular vesicles. The approach is scalable and employs relatively gentle manipulation forces to fractionate and concentrate extracellular vesicles with good purity and functional integrity.
10#
發(fā)表于 2025-3-23 06:06:15 | 只看該作者
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