Titlebook: Expression, Purification, and Structural Biology of Membrane Proteins; Camilo Perez,Timm Maier Book 2020 Springer Science+Business Media,

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Image and Video-Based Artistic Stylisationsion of different gene products. The expressed protein engineered with a suitable tag can be readily detected and purified from crude membrane extracts. The purpose of this protocol chapter is to detail the procedures of cloning, expression, isolation of the membrane vesicles, and affinity purification of a membrane protein of interest in

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Computer-Aided Detection of Lung Cancerof the final protein sample. Here, we describe several protocols for affinity purification of TSPO, a small membrane protein. The techniques we use include immobilized metal affinity chromatography (IMAC) and strep-II tag-based streptavidin affinity chromatography.

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