Titlebook: Expressed Sequence Tags (ESTs); Generation and Analy John Parkinson Book 2009 Humana Press 2009 Alternative transcripts.Bur.Expression prof

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書目名稱Expressed Sequence Tags (ESTs)影響因子(影響力)




書目名稱Expressed Sequence Tags (ESTs)影響因子(影響力)學(xué)科排名




書目名稱Expressed Sequence Tags (ESTs)網(wǎng)絡(luò)公開度




書目名稱Expressed Sequence Tags (ESTs)網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Expressed Sequence Tags (ESTs)被引頻次




書目名稱Expressed Sequence Tags (ESTs)被引頻次學(xué)科排名




書目名稱Expressed Sequence Tags (ESTs)年度引用




書目名稱Expressed Sequence Tags (ESTs)年度引用學(xué)科排名




書目名稱Expressed Sequence Tags (ESTs)讀者反饋




書目名稱Expressed Sequence Tags (ESTs)讀者反饋學(xué)科排名

TIA742

Strategies for Undertaking Expressed Sequence Tag (EST) Projects,rposes. They are especially important for enhancing the utility of a genome sequence or for providing a gene catalog for a genome that has not or will not be sequenced. In planning and executing a cDNA project, several criteria must be considered. One should clearly define the project purpose, inclu

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CLAY

Preparation of Full-Length cDNA Libraries: Focus on Metazoans,btraction of redundant transcripts. The use of trehalose and sorbiol stabilizes the activity of the reverse transcriptase leading to efficient cDNA synthesis and the cap-trapping method is used for efficient full-length cDNA selection. Through the incorporation of additional normalization and subtra

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出來(lái)

Generating EST Libraries: ,-Spliced cDNAs,r a fraction) of their mRNAs. This feature may be exploited for the preparation of full-length-enriched cDNA libraries from these organisms (a diverse group including euglenozoa and dinoflagellates, as well as members from five metazoan phyla: Cnidaria, Rotifera, Nematoda, Platyhelminths and Chordat

CANDY

Large-Scale Sequencing and Analytical Processing of ESTs,, their multipurpose uses, such as in probe design for microarrays, determining alternative splicing, verifying open reading frames, and confirming exon/intron and gene boundaries, to name a few, further justify their inclusion in many genomic characterization projects. Hence, there has been a const

liaison

EST Processing: From Trace to Sequence,bjects. Here we describe the usage of a software pipeline (available from .), which has been developed to make the most of EST datasets. This modular software solution is targeted toward small- to medium-sized EST projects and comprises a series of Perl scripts. The software design is based on our e

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