Titlebook: Expressed Protein Ligation; Methods and Protocol Miquel Vila-Perelló Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Identification, Characterization, and Optimization of Split Inteins, the protein splicing reaction, information that can guide further engineering efforts. These techniques provide useful alternatives to common assays that utilize SDS-PAGE to analyze splicing reaction progress.

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,Die innere Uhr - Was l?sst uns ticken?,N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the action of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein li

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Maarten J. Arentsen,Doris Fuchsession. Peptide hydrazides are widely used as thioester equivalents in the total chemical synthesis and semisynthesis of proteins as they can be easily prepared using standard solid phase peptide synthesis (SPPS) and recombinant peptide techniques. Via treatment with NaNO. and subsequent thiolysis,

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https://doi.org/10.1007/978-3-663-20507-4ir ultrafast splicing kinetics allow for the efficient production of native proteins from two separate polypeptides both in vitro and in cells. They can also be used to generate proteins with C-terminal thioesters for downstream applications. In this chapter, we describe a method based on a genetica