Titlebook: Experiments in Molecular Biology; Robert J. Slater Book 1986 Springer Science+Business Media LLC 1986 biology.Colon.DNA.enzyme.enzymes.Esc

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Detection of Plasmid by Colony Hybridization, by considering the following problem. A scientific group wishes to clone in bacteria a particular human chromosomal gene. There would be no difficulty obtaining human DNA, cutting it with a restriction endonuclease, and ligating the many fragments obtained into vector molecules. This ligation mix c

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Isopycnic Centrifugation of DNA,ually associated. Cells of plants and animals contain from 1 to 100 pg of DNA per nucleus. This does not sound like a lot until one realizes that is represents 100–10,000 times as much DNA as in an . chromosome. When faced with such vast complexity, it is obviously an advantage if the DNA can be sep

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Purification of Plant Ribosomal DNA,sed in the preceeding chapter. This chapter describes a modification of the procedure that enhances the separation of specific DNA sequences from main-band DNA. The method is based on the fact that actinomycin D binds preferentially to DNA molecules with a high-GC content. This . their density relat

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The Extraction and Affinity Chromatography of Messenger RNA,A molecules are involved in the expression of the genetic material (DNA) and are, in fact, the initial products of gene expression that go on to direct protein synthesis. Knowledge of the structure, metabolism, and mode of action of RNA in protein synthesis is therefore fundamental to our understand

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Agarose Gel Electrophoresis of RNA, simple, but nevertheless achieves very reproducible results and high resolution. RNA is a polyanion and will therefore migrate toward the positive electrode in an electric field. If the migration occurs through a gel matrix of carefully chosen pore size, the mobility of the RNA molecules is related

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In Vitro Protein Synthesis,h prokaryotic and eukaryotic systems have been developed, but for this experiment only a eukaryotic system will be considered. In 1973, Roberts and Paterson (.) demonstrated that a cell-free extract of wheat germ would faithfully translate rabbit globin mRNA and tobacco mosaic virus RNA to yield ful

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