Titlebook: Exocytosis and Endocytosis; Methods and Protocol Florence Niedergang,Nicolas Vitale,Stéphane Gasman Book 2021 Springer Science+Business Med

floodgate

puzzle

,Oberfl?chengl?ttung von Lagenh?lzern,ion of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.

有毛就脫毛

不適當(dāng)

柔聲地說

Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocyticen widely exploited to study constitutive endocytosis or ligand-induced receptor endocytosis. Compensatory endocytosis is the mechanism by which components of secretory vesicles are retrieved after vesicle fusion with the plasma membrane in response to cell stimulation and a rise in intracellular ca

遺留之物

他姓手中拿著

High-Content Drug Discovery Screening of Endocytosis Pathwaysms, classified depending on critical proteins involved, speed, morphology of the derived intracellular vesicles, or substance trafficked. Pharmacological targeting of specific endocytosis pathways has a proven utility for diverse clinical applications from epilepsy to cancer. A multiplexable, high-c

上流社會(huì)

Methods for Monitoring Endocytosis in Astrocytesnism linked to different types of endocytosed cargo, including pathogens; therefore, several approaches can be applied. Here, we describe techniques that are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signal

Obsessed

Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent D, the dominant mechanism of SV retrieval is activity-dependent bulk endocytosis (ADBE). Here, we describe a method to monitor ADBE in isolation from other SV endocytosis modes, via the uptake of large fluorescent fluid-phase markers in primary neuronal culture. Furthermore, we outline how to monitor

conspicuous

Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretionensitive to changes in cell number, and a significant amount of effort has to be spent to overcome this. We have developed a panel of quantitative flow cytometry-based assays and reporter cell lines that can be used to measure constitutive secretion. These assays are insensitive to changes in cell n