Titlebook: Exocytosis and Endocytosis; Methods and Protocol Florence Niedergang,Nicolas Vitale,Stéphane Gasman Book 2021 Springer Science+Business Med

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Super-Resolution Microscopy and Particle-Tracking Approaches for the Study of Vesicular Trafficking hil vesicular trafficking, exocytosis, and associated functions at the molecular level. Here, we describe super-resolution and total internal reflection fluorescence (TIRF) microscopy approaches for the analysis of vesicular trafficking and associated functions of primary neutrophils.

BROTH

An Approach to Monitor Exocytosis in White Adipocytesion of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.

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https://doi.org/10.1007/978-3-658-23345-7ariability in the assay. Using this approach in 384-well format, at least 48 and as many as 144 plates can theoretically be processed per week. This protocol serves as a guideline and can be modified as required for alternate stimulation paradigms and improved upon as new technologies become available.

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,Coomologia Sulle Varieta’ Complesse, I.,ay based on the successive transformation of adrenaline into adrenochrome and adrenolutine for fluorescence measurements. We also provide some guidelines for efficient cell recovery and for the use of this assay in the laboratory.

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High-Throughput Screening for Insulin Secretion Modulatorsariability in the assay. Using this approach in 384-well format, at least 48 and as many as 144 plates can theoretically be processed per week. This protocol serves as a guideline and can be modified as required for alternate stimulation paradigms and improved upon as new technologies become available.

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Bovine Chromaffin Cells: Culture and Fluorescence Assay for Secretionay based on the successive transformation of adrenaline into adrenochrome and adrenolutine for fluorescence measurements. We also provide some guidelines for efficient cell recovery and for the use of this assay in the laboratory.