Titlebook: Ewing Sarcoma; Methods and Protocol Florencia Cidre-Aranaz,Thomas G. P. Grünewald Book 2021 Springer Science+Business Media, LLC, part of S

同位素

Russell Wanhill,Simon Barter,Loris Molentor future genomic investigations of EwS. We anticipate this recommended analytical framework for germline and somatic investigations, along with genomic data from growing EwS case series, will aid in accelerating new genomic discoveries in EwS and expand knowledge of the genetic architecture of EwS.

dithiolethione

Fatigue and Fracture Reliability Engineeringis chapter we describe a RT-PCR method that allows for the detection of the most frequent alterations with elevated specificity and sensitivity which is able to distinguish among the different types of fusions. The method is fast and economical, and can be carried out with the conventional equipment available in any molecular biology laboratory.

種族被根除

https://doi.org/10.1007/978-3-319-56227-8veral fusion transcripts simultaneously with previous knowledge of only one partner gene. Here we describe in detail our protocol and tips for nucleic acid extraction, library preparation, sequencing, and reporting of gene fusions.

不幸的人

acclimate

https://doi.org/10.1007/978-3-031-13498-2tal assessment of proliferation in preclinical research plays an important role. Among the variety of applicable methods, trypan blue exclusion is described here as a robust, easy-to-perform, and cost-effective method to assess cell proliferation in an experimental setting.

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植物學(xué)

harbinger

誘惑

Analysis of Regulatory DNA Sequences by Dual-Luciferase Reporter Assays in Ewing Sarcomase reporter assays in Ewing sarcoma cell lines. To this end, we provide a protocol for cloning sequences of interest from genomic DNA into a firefly luciferase-containing plasmid, transfecting Ewing sarcoma cells with plasmids and measuring luciferase expression by luminescence. The entire procedure can be completed in 14?days.