Titlebook: Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation; Narendra Wajapeyee,Romi Gupta Book 2017 Springer Science+B

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https://doi.org/10.1007/978-0-387-75843-5ther cell types. We describe here a detailed protocol for the isolation of highly purified endocrine cell subtypes (beta, alpha, and delta cells) from human cadaveric islets, to perform cell-type-specific epigenomic analysis of histone modifications as well as global gene expression profiling.

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Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets,ther cell types. We describe here a detailed protocol for the isolation of highly purified endocrine cell subtypes (beta, alpha, and delta cells) from human cadaveric islets, to perform cell-type-specific epigenomic analysis of histone modifications as well as global gene expression profiling.

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Book 2017y-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,?.Eukaryotic Transcription and Post-Transcription Gene Expression Regulation?.aims to ensure successful results in the further study of this vital field..

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The , as Transcendental Phenomenologyoid, dependence on creatine phosphate. Important reaction components needed for optimal processing are discussed. We also provide an optimized procedure for preparing small-scale HeLa nuclear extracts from adherent cells for use in 3′ cleavage in vitro.

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https://doi.org/10.1057/9780230283978 stable human embryonic stem cell line containing both Sp-dCas9-KRAB and sgRNA, followed by inducible expression of Sp-dCas9-KRAB to analyze functional effects of dCas9-KRAB at target loci in human embryonic stem cells.

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Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human stable human embryonic stem cell line containing both Sp-dCas9-KRAB and sgRNA, followed by inducible expression of Sp-dCas9-KRAB to analyze functional effects of dCas9-KRAB at target loci in human embryonic stem cells.