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Titlebook: Erythropoiesis; Methods and Protocol Joyce A. Lloyd Book 2018 Springer Science+Business Media LLC 2018 Animal models.Red blood cells.Progen

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發(fā)表于 2025-3-21 17:21:05 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Erythropoiesis
副標(biāo)題Methods and Protocol
編輯Joyce A. Lloyd
視頻videohttp://file.papertrans.cn/316/315093/315093.mp4
概述Includes cutting-edge methods and protocols for the study of erythropoiesis.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Erythropoiesis; Methods and Protocol Joyce A. Lloyd Book 2018 Springer Science+Business Media LLC 2018 Animal models.Red blood cells.Progen
描述This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Erythropoie
出版日期Book 2018
關(guān)鍵詞Animal models; Red blood cells; Progenitor cell status; Erythroid cells; Chromatin immunoprecipitation (
版次1
doihttps://doi.org/10.1007/978-1-4939-7428-3
isbn_softcover978-1-4939-8483-1
isbn_ebook978-1-4939-7428-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2018
The information of publication is updating

書目名稱Erythropoiesis影響因子(影響力)




書目名稱Erythropoiesis影響因子(影響力)學(xué)科排名




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書目名稱Erythropoiesis網(wǎng)絡(luò)公開度學(xué)科排名




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Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands,and maintain the integrity of the niche. To improve our understanding of the molecular regulation of erythroid cells in EBIs, we have developed protocols for immuno-gold labeling of erythroid surface antigens to combine with scanning electron microscopy. These protocols have allowed imaging of EBIs
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Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells,t is possible to unambiguously distinguish erythroblasts at each developmental stage during murine terminal erythroid differentiation. We also developed methods for the analysis and isolation of human erythroid cells at all developmental stages. BFU-E and CFU-E are characterized by CD45.GPA.IL-3R.CD
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High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure,unofluorescence staining and confocal imaging of various molecules, organelles and structures of interest in erythroblasts, and to present and quantitatively analyze the data obtained in these fluorescence images.
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發(fā)表于 2025-3-23 02:49:43 | 只看該作者
Book 2018Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Erythropoie
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發(fā)表于 2025-3-23 05:35:48 | 只看該作者
,China’s Rural Economic Restructuring,ery useful cell lines have been developed by manipulating ES or fetal liver erythroid progenitor cells from knockout mouse models. In recent years, our understanding of erythropoiesis has improved, due to the ability to knock down genes in native human hematopoietic stem and progenitor cells derived
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