Titlebook: ErbB Receptor Signaling; Methods and Protocol Zhixiang Wang Book 2017 Springer Science+Business Media LLC 2017 Cell signaling.Epidermal gro

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diabetes

Devastate

Ryan Holmes,Darren McCauley,Nick HanleyG1478 and monensin causes endosome-associated EGFR activation. Phosphorylated surface EGFR levels are undetectable during the treatment. This is a unique and novel approach to study endosomal signaling and its physiological relevance. The protocol is transferable to study endosomal signaling of other receptor tyrosine kinases.

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Methods to Investigate EGFR Ubiquitinationd reliable method to assess EGFR ubiquitination from low amount of sample; lastly, we illustrate an immunofluorescence protocol to visualize ubiquitinated species (including the EGFR itself) within the EGFR-positive endocytic compartments upon EGF stimulation.

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Activation of Endosome-Associated Inert EGF Receptor Following InternalizationG1478 and monensin causes endosome-associated EGFR activation. Phosphorylated surface EGFR levels are undetectable during the treatment. This is a unique and novel approach to study endosomal signaling and its physiological relevance. The protocol is transferable to study endosomal signaling of other receptor tyrosine kinases.