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Titlebook: Epstein-Barr Virus Protocols; Joanna B. Wilson,Gerhard H. W. May Book 2001 Humana Press 2001

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書(shū)目名稱(chēng)Epstein-Barr Virus Protocols
編輯Joanna B. Wilson,Gerhard H. W. May
視頻videohttp://file.papertrans.cn/314/313403/313403.mp4
概述Includes supplementary material:
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Epstein-Barr Virus Protocols;  Joanna B. Wilson,Gerhard H. W. May Book 2001 Humana Press 2001
描述The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702–703), was stimulated by Denis Burkitt’s rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late
出版日期Book 2001
版次1
doihttps://doi.org/10.1385/1592592279
isbn_softcover978-1-61737-137-0
isbn_ebook978-1-59259-227-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2001
The information of publication is updating

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Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell LeveNA migrate with known and different mobilities, the Gardella gel (.), and combined it with a highly sensitive DNA polymerase chain reaction (PCR) system (.,., and . in this volume), that can be used to detect a single copy of the viral genome in 10. uninfected cells. The result is a technique that c
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Virus Isolationne (.) and the Akata cell line (.). B95-8 cells and P3HR1 cells are usually induced with phorbol esters, sodium butyrate, or a mixture of the two (.). In addition, replication can be induced in the Akata cell line by crosslinking cell surface immunoglobulin (.).
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Transient Gene Expression and MACS Enrichmentociated with a selection process (.,.). This selection process prevents the analysis of early effects of expressed viral genes and could also lead to secondary cell culture effects not associated with the viral gene. Often, inducible gene expression systems are difficult to modulate and can be leaky
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1064-3745 enic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late978-1-61737-137-0978-1-59259-227-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Book 2001arch, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late
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