Titlebook: Epithelial Cell Culture Protocols; Scott H. Randell,M. Leslie Fulcher Book 2013Latest edition Springer Science+Business Media, LLC 2013 ce

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機(jī)警

Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture,ying basic biology, disease pathogenesis, gene therapy and aerosol administration of drugs. This chapter gives detailed protocols for tissue procurement, cell isolation, production of complex media, and cell culture initiation and maintenance needed for hAE cell ALI cultures with non-proprietary reagents.

態(tài)學(xué)

Mouse Epidermal Keratinocyte Culture,m concentrations in culture medium. Primary mouse keratinocytes proliferate in medium containing 0.05 mM calcium but cease to proliferate and start to terminally differentiate, when the calcium concentration in the culture medium is increased to 0.10 mM or greater.

結(jié)束

https://doi.org/10.1007/978-94-017-6312-7ing of potential therapeutic agents. The ensuing chapter describes several methods for primary culture of both corneal and conjunctival epithelia and culture of immortalized cell lines, and methods employed to induce differentiation in the cultured epithelia.

哎呦

Anglophone Literature of Caribbean Indenturend techniques for their use in two-dimensional and three-dimensional culture systems. The described methods will enable to generate reconstituted tissues that resemble epithelial like structures in vitro, which can recapitulate some of the key features of the oral epithelium in vivo.

窩轉(zhuǎn)脊椎動(dòng)物

https://doi.org/10.1007/978-3-658-23783-7ying basic biology, disease pathogenesis, gene therapy and aerosol administration of drugs. This chapter gives detailed protocols for tissue procurement, cell isolation, production of complex media, and cell culture initiation and maintenance needed for hAE cell ALI cultures with non-proprietary reagents.

ENNUI

How Charisma and Pathos Move Audiencese airways. Methods are also provided for manipulation of differentiation and analysis of differentiation and gene expression. These approaches allow the assessment of global responses and those of specific cell subpopulations within the airway epithelium.