Titlebook: Epithelial Cell Culture; Methods and Protocol Mario Baratta Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 ca

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Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Pepithelial primary cultures containing mucus cells, neck cells, parietal cells, chief cells, and gastrin-secreting endocrine cells. This simple and convenient method will prove useful in the isolation of normal human gastric mucous epithelial cells for in vitro studies of gastric epithelial biology.

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Long-Term Culture of Intestinal Organoids,cryopreservation and defining characteristics of these intestinal organoids also make this work relevant for the advancement of epithelial organoid-based therapeutic technologies by allowing the production of large numbers of cells for use in clinical applications.

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https://doi.org/10.1007/978-3-319-28140-7 intestinal mucosa requires therefore further investigation, particularly focused in addressing the abovementioned unmet needs. 3D models came as bridge between the in vitro and in vivo models. These models are proven to be influential of the drug effect in cells, being the most adequate to mimic th

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Auguste Wackenheim M.D.,Jean-Paul Braun M.D. vivo characteristics, such as different cell types and complex tissue architecture. This in vitro (ex vivo. organ culture protocol of the swine large intestine aims at maintaining viable colonic mucosa for up to 5?days. The protocol described herein applies a combination of methods used for immorta

教唆

Reassembly of Functional Human Stem/Progenitor Cells in 3D Culture,diation xerostomia using a “bottom up” reassembly paradigm. We use specialized extracellular matrix and growth factor supplemented hyaluronate hydrogels to promote reassembly of human salivary stem/progenitor cells (hS/PCs) isolated after surgical resection, a method we describe in this chapter. Cel

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