Titlebook: Enzymes of Molecular Biology; Michael M. Burrell Book 1993 Humana Press 1993

STALL

The BAL 31 Nucleases (EC 3.1.11),e shown (.,.) to be bacterial products. Only 10–20% of the nuclease activity is found in the periplasm (.). The American Type Culture Collection strain of . (ATCC 29659) produces BAL 31 nuclease as proficiently as the strain originally obtained from its discoverer.

血友病

BLOT

Pronase (EC 3.4.24.4),used, for example, to reveal the protein components of cell organelles by the hydrolysis of tissue slices (.), and as an alternative to proteinase K to remove protein during plasmid DNA (.), chromosomal DNA (.), and RNA isolation (.–.). Another use of pronase is the production of a protein hydrolysate suitable for amino acid analysis (.,.).

avarice

Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1),lar biology, The Type II restriction endonucleases obviously fall into this category, however they are a large subject on their own and therefore are dealt with in a separate section of this book. DNases acting preferentially on single-stranded DNA (ss DNA) substrates are also dealt with in another section of this chapter.

不可接觸

惰性女人

COW

亞麻制品

cumulative

Outlook: Agile as the New Normal?,homononucleotides. This chapter focuses on the endoribonuclease RNase A (otherwise described as RNase, RNase I, or pancreatic ribonuclease), which shows some base specificity in where it cleaves RNA. The enzyme has been particularly well characterized at the molecular level.

雄辯

Agile in der Unternehmenspraxisused, for example, to reveal the protein components of cell organelles by the hydrolysis of tissue slices (.), and as an alternative to proteinase K to remove protein during plasmid DNA (.), chromosomal DNA (.), and RNA isolation (.–.). Another use of pronase is the production of a protein hydrolysate suitable for amino acid analysis (.,.).