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Titlebook: Enzyme-Mediated Immunoassay; T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol

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發(fā)表于 2025-3-23 12:05:05 | 只看該作者
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Eyewitness Identification by Theft Victims,in contrast to competitive radioimmunoassays or enzyme-immunoassays in which labeled-antigens are utilized in competition with sample antigen to bind to a limited number of antibody sites. The label applied to the antibody can take the form of a radioisotope, enzyme, fluorophore, luminescent tag, or
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發(fā)表于 2025-3-24 05:16:50 | 只看該作者
Palgrave Macmillan Memory Studiesmaleimide derivatives have been used for coupling the enzyme to proteins, such as immunoglobulins and their fragments (Ishikawa, 1983). Use of these coupling agents showed no appreciable reduction of enzyme activity due to the involvement of sulfhydryl groups of the enzyme in the coupling process. C
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https://doi.org/10.1057/9780230305137We describe here an approach to separation-free enzyme immunoassays based on the ability of specific antibodies to ligands to restrict the conformational mobility of an apoenzyme that has already been labeled with that ligand, to fold into a catalytically active conformation.
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