Titlebook: Environmental Microbiology; Methods and Protocol Ian T. Paulsen,Andrew J. Holmes Book 2014Latest edition Springer Science+Business Media, L

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Quantitative PCR for Detection of mRNA and gDNA in Environmental Isolatesation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative P

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Terminal Restriction Fragment Length Polymorphism (T-RFLP) Profiling of Bacterial 16S rRNA Genessity can be generated in a sensitive, reproducible, and cost-effective manner. This protocol describes the steps required to generate T-RFLP profiles of the dominant members of a bacterial community, by PCR amplification of the bacterial 16S rRNA genes and three restriction endonuclease digests to g

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Biolog Phenotype MicroArrays for Phenotypic Characterization of Microbial Cellsimetric reaction that is indicative of respiration, these microplate assays measure the response of an individual strain or microbial community to a large and diverse range of nutrients and chemicals. Phenotype MicroArrays have been used to study gene function and to improve genome annotation in sin

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