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Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut

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發(fā)表于 2025-3-21 16:17:23 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Enhancers and Promoters
副標題Methods and Protocol
編輯Tilman Borggrefe,Benedetto Daniele Giaimo
視頻videohttp://file.papertrans.cn/312/311271/311271.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut
描述.This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe?enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays.?Written in the .Methods in Molecular Biology?.series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting..Authoritative and cutting-edge,?.Enhancers and Promoters: Methods and Protocols.?is a useful guide for future experiments..Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
出版日期Book 2021
關鍵詞inflammation; Myc locus; Histone; eRNAs; T-Cells
版次1
doihttps://doi.org/10.1007/978-1-0716-1597-3
isbn_softcover978-1-0716-1599-7
isbn_ebook978-1-0716-1597-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Global Run-on Sequencing (GRO-Seq) RNA, such as mRNAs, rarely reflect accurately the rate of in situ transcription in nuclei by RNA polymerases (RNAPs). The “Global Run-on Sequencing (GRO-Seq)” method, developed in 2008, combines the nuclear run-on assay with next-generation deep sequencing to detect nascent RNA levels to annotate t
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Low Input Targeted Chromatin Capture (Low-T2C)ed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinf
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Analysis of Enhancer–Promoter Interactions using CAGE and RADICL-Seq Technologiesrch. Enhancer–promoter interactions are believed to mediate activation of target genes. Bidirectional transcription represents one hallmark of active enhancers that can be measured using transcriptome technologies such as Cap analysis of gene expression (CAGE). Recently, we have developed RNA and DN
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