Titlebook: Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions; Ang Yan Shan Book 2018 Springer Nature Singapore P

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Angela Wigger,Hubert Buch-Hansenide mutations in DNA and RNA targets, aptamers to characterize thrombin binding, and antibodies to detect cell surface receptor dimers. We demonstrated through these studies that the split proximity circuit can be used for varied purposes, from simple end-point detection to semi-quantitative analysi

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Introduction,s was achieved by designing a target-responsive, self-contained DNA circuit to execute localized strand displacement reactions (Chaps. .–.) while minimizing circuit leakage through thermodynamics-driven design concepts (Chaps. . and .). We then demonstrated the applicability of the designed circuit,

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Modular Framework for Engineering a Self-contained DNA Circuit,concentration (50 nM–5 μM) in a single mixing step without any further washing. The formation of localized signal at the interaction site can be enhanced kinetically through the control of temperature and probe concentration. This work provides a basic general framework from which other circuit modu