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Titlebook: Engineered Zinc Finger Proteins; Methods and Protocol Joel P. Mackay,David J. Segal Book 2010 Springer Science + Business Media, LLC 2010 A

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書目名稱Engineered Zinc Finger Proteins
副標題Methods and Protocol
編輯Joel P. Mackay,David J. Segal
視頻videohttp://file.papertrans.cn/311/310687/310687.mp4
概述Chapters from nearly all of the top research groups in the field.Sections on zinc finger nucleases, one of the most exciting tools for gene therapy and genome engineering in the past decade.Includes s
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Engineered Zinc Finger Proteins; Methods and Protocol Joel P. Mackay,David J. Segal Book 2010 Springer Science + Business Media, LLC 2010 A
描述Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins.In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives.The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects.Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital met
出版日期Book 2010
關(guān)鍵詞Artificial transcription factors; DNA; DNA binding domains; Designer ZFPs; ZFP construction; Zinc finger
版次1
doihttps://doi.org/10.1007/978-1-60761-753-2
isbn_softcover978-1-4939-5751-4
isbn_ebook978-1-60761-753-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science + Business Media, LLC 2010
The information of publication is updating

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Quickoffice and Quickoffice Pro,e display for selecting zinc fingers with desired specificities from such libraries. In this chapter we provide updated, detailed protocols for performing zinc finger selections using the bacterial two-hybrid system.
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Touching Your Photos and Videos,table repression of known target genes. We review the literature on targeted DNA methylation and gene silencing, summarize the achievements and the challenges that remain, and discuss technical issues critical for this approach.
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Book 2010teins.In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives.The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and de
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Erica Sadun,Michael Grothaus,Steve Sandeng specificities applied to C.H. zinc finger proteins, based on FoldX, a semiautomatic protein design tool. Given a template structure, this method generates candidate mutants for a given target DNA sequence selected by energetic criteria.
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Taking Your iPhone 4 to the Maxolymerase across the ZFP-binding region. Taking into account some design considerations, this method of inhibiting viral transcription can be applied to other viral infections where viral dsDNA occurs.
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