Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author

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Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction Sysd applications in developmental biology, related developmental disease modeling, and drug discovery. Several cell resources have been utilized, with different efficiencies and methods for generating human blastoids, a structure similar to natural blastocysts. Human EPS cells were reported to contrib

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Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Techlly gene editing and transcriptional regulation technology. Human trophoblast stem cell (hTSC) is a promising cell model for simulating the development and function of human placental trophoblast cells. Here, we provide an optimized workflow for efficient genome editing and transcriptional activatio

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,Generation of 3D Trophoblast Organoids from Human Na?ve Pluripotent Stem Cells,ells found within the placenta and comprise a variety of distinct cell types with specialized roles in fetal-maternal communication. Our understanding of human trophoblast development remains limited due to ethical and legal restrictions on accessing first-trimester placental tissues, as well as the

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Dorsal-Kyphosis