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Titlebook: Elispot for Rookies (and Experts Too); Sylvia Janetzki Book 2016 Springer International Publishing Switzerland 2016 B-cell Assay.Elispot.F

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發(fā)表于 2025-3-21 19:11:36 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Elispot for Rookies (and Experts Too)
編輯Sylvia Janetzki
視頻videohttp://file.papertrans.cn/308/307703/307703.mp4
概述Provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible.Allows the novice user to truly understand the best options for specific protocol steps, reagen
叢書名稱Techniques in Life Science and Biomedicine for the Non-Expert
圖書封面Titlebook: Elispot for Rookies (and Experts Too);  Sylvia Janetzki Book 2016 Springer International Publishing Switzerland 2016 B-cell Assay.Elispot.F
描述.This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators..The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation..
出版日期Book 2016
關(guān)鍵詞B-cell Assay; Elispot; Flurospot; Immune Monitoring; T-cell Assay
版次1
doihttps://doi.org/10.1007/978-3-319-45295-1
isbn_softcover978-3-319-83260-9
isbn_ebook978-3-319-45295-1Series ISSN 2367-1114 Series E-ISSN 2367-1122
issn_series 2367-1114
copyrightSpringer International Publishing Switzerland 2016
The information of publication is updating

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發(fā)表于 2025-3-21 21:29:16 | 只看該作者
Techniques in Life Science and Biomedicine for the Non-Experthttp://image.papertrans.cn/e/image/307703.jpg
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發(fā)表于 2025-3-22 01:28:17 | 只看該作者
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發(fā)表于 2025-3-22 07:36:50 | 只看該作者
Elispot for Rookies (and Experts Too)978-3-319-45295-1Series ISSN 2367-1114 Series E-ISSN 2367-1122
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發(fā)表于 2025-3-22 09:18:05 | 只看該作者
Methoden der Marketingforschung (Aufgaben),Conducting an Elispot assay is a multistep process. The main steps are:
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發(fā)表于 2025-3-22 15:32:15 | 只看該作者
https://doi.org/10.1007/978-3-322-92052-2The choice of the Elispot plate can dramatically influence the final results of an Elispot experiment. Membrane-based 96-well Elispot plates are the optimal choice for Elispot. The membrane fibers look like a tightly woven web of polymers that offers a large surface for protein binding (Fig. 3.1).
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發(fā)表于 2025-3-23 01:43:23 | 只看該作者
Gianfranco Walsh,David Dose,Maria SchwabeAs an important resource, a step-by-step Elispot protocol with important annotations is provided elsewhere [79].
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發(fā)表于 2025-3-23 09:18:29 | 只看該作者
https://doi.org/10.1007/978-3-658-11649-1Throughout the book we have touched upon various important controls, for the sample (apoptosis and viability, see Sect. 4.6), and for the assay (negative controls, sample-specific internal controls, nonspecific positive controls, external controls, see Sect. 5.1).
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