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Titlebook: Electroporation and Electrofusion in Cell Biology; Eberhard Neumann,Arthur E. Sowers,Carol A. Jordan Book 1989 Springer Science+Business M

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書目名稱Electroporation and Electrofusion in Cell Biology
編輯Eberhard Neumann,Arthur E. Sowers,Carol A. Jordan
視頻videohttp://file.papertrans.cn/307/306531/306531.mp4
圖書封面Titlebook: Electroporation and Electrofusion in Cell Biology;  Eberhard Neumann,Arthur E. Sowers,Carol A. Jordan Book 1989 Springer Science+Business M
描述Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
出版日期Book 1989
關(guān)鍵詞Cytosol; Expression; Lipid; Mammalia; cell biology; fluorescence; gene transfer; microorganism
版次1
doihttps://doi.org/10.1007/978-1-4899-2528-2
isbn_softcover978-1-4899-2530-5
isbn_ebook978-1-4899-2528-2
copyrightSpringer Science+Business Media New York 1989
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https://doi.org/10.1007/978-1-349-25434-7the study of the effect of PEF on cell membranes has spanned more than three decades (Cole, 1972; see also Schwan, this volume). The present chapter reviews the physical characteristics of cell membranes that are essential for understanding the electroporation phenomenon and summarizes experiments d
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Dielectrophoresisand Crane, 1971); the behavior of living and nonliving cells (Pohl, 1978; Iglesias ., 1984); and the response of cellular organelles (Ting ., 1971). The application of dielectrophoresis to biological materials is also useful in analyzing phenomena of natural electric oscillation (Pohl, 1983), cell r
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Book 1989dding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
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