| 書目名稱 | Electroporation Protocols for Microorganisms | | 編輯 | Jac A. Nickoloff | | 視頻video | http://file.papertrans.cn/307/306530/306530.mp4 | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro- organisms describe the underlying theory of electroporation, the com- mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre- sents a well developed method for electrotransformation of a particular bacterial, fungal, o | | 出版日期 | Book 1995 | | 版次 | 1 | | doi | https://doi.org/10.1385/0896033104 | | isbn_softcover | 978-0-89603-310-8 | | isbn_ebook | 978-1-59259-534-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 1995 |
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