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Titlebook: Electrophoretic Separation of Proteins; Methods and Protocol Biji T. Kurien,R. Hal Scofield Book 2019 Springer Science+Business Media, LLC,

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51#
發(fā)表于 2025-3-30 09:08:29 | 只看該作者
Introduction to Protein Electrophoresis,This chapter briefly discusses the developments in electrophoresis of proteins from Tiselius’ moving-boundary electrophoresis to the modern-day two-dimensional polyacrylamide gel electrophoresis. It also touches upon the staining methods used to visualize total proteins post electrophoresis.
52#
發(fā)表于 2025-3-30 16:20:26 | 只看該作者
53#
發(fā)表于 2025-3-30 18:16:09 | 只看該作者
54#
發(fā)表于 2025-3-30 21:47:54 | 只看該作者
55#
發(fā)表于 2025-3-31 01:47:20 | 只看該作者
Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis,etect, identify, and analyze these posttranslational modifications. This new cost-effective methodology will aid the detection of unwanted glycation products in processed foods and may lead to new diagnostics and therapeutics for age-related chronic diseases.
56#
發(fā)表于 2025-3-31 07:26:28 | 只看該作者
57#
發(fā)表于 2025-3-31 10:23:16 | 只看該作者
58#
發(fā)表于 2025-3-31 16:44:25 | 只看該作者
Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Esolubilization of heat shock proteins (HSP) B1 and B5 and the cytoplasmic adapter protein integrin-linked kinase (ILK) from smooth muscle. Overall, the results demonstrate the importance of optimizing lysis buffers for specific protein solubilization prior to finalizing the experimental workflow.
59#
發(fā)表于 2025-3-31 18:38:51 | 只看該作者
60#
發(fā)表于 2025-4-1 00:47:25 | 只看該作者
Isoelectric Focusing on Non-Denaturing Flatbed Gels,ted over several years were used for analysis using flatbed IEF. Following electrofocusing, the gel is analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane to determine oligoclonality of the anti-Ro 60 containing sera.
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