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Titlebook: Electron Probe Microanalysis; Applications in Biol Karl Zierold,Herbert K. Hagler (Associate Professo Conference proceedings 1989 Springer-

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發(fā)表于 2025-3-21 16:43:08 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Electron Probe Microanalysis
副標(biāo)題Applications in Biol
編輯Karl Zierold,Herbert K. Hagler (Associate Professo
視頻videohttp://file.papertrans.cn/307/306145/306145.mp4
叢書名稱Springer Series in Biophysics
圖書封面Titlebook: Electron Probe Microanalysis; Applications in Biol Karl Zierold,Herbert K. Hagler (Associate Professo Conference proceedings 1989 Springer-
描述The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti- cular diffusible ions (elements). 2. Electron probe microanaly- sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con- verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo- gical processes requires sampling techn
出版日期Conference proceedings 1989
關(guān)鍵詞X-ray; cells; diagnosis; spectroscopy; tissue
版次1
doihttps://doi.org/10.1007/978-3-642-74477-8
isbn_softcover978-3-642-74479-2
isbn_ebook978-3-642-74477-8Series ISSN 0932-2353 Series E-ISSN 1868-2561
issn_series 0932-2353
copyrightSpringer-Verlag Berlin Heidelberg 1989
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 21:11:09 | 只看該作者
0932-2353 tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used
板凳
發(fā)表于 2025-3-22 02:42:00 | 只看該作者
Homeostasis - Tumor - Metastasisnalysis and a review of the categories of biological studies and of the notable biological results (for example, see Hall, 1979.) Instead, here we shall try to trace the actual path of development of the method, a development shaped of course by the interaction of all of these aspects as the available instrumentation became ever more effective.
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Specimen Preparation and Other Limitations in Quantitative Eletron Probe X-Ray Microanalysis (EPXMA)terfere with the logic of the study at all. Conventional specimen preparation methods have been used to characterize, for example, asbestos fibres in lung tisue (Champness et al., 1976) or the accumulation of heavy metals in kidney tissue (Galle et al., 1968).
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Homer in der deutschen LiteraturThe conformation of any macromolecule in a given cell is primarily determined by the interplay of internal and external electric charges. Local water content and ion concentrations are basic regulators of all cellular functions, therefore.
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Springer Series in Biophysicshttp://image.papertrans.cn/e/image/306145.jpg
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978-3-642-74479-2Springer-Verlag Berlin Heidelberg 1989
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