Titlebook: Eicosanoid Protocols; Elias A. Lianos Book 1999 Humana Press 1999

闡釋

https://doi.org/10.1007/978-3-658-15040-2 obtain stable expression of the exogenous gene. Viral vectors, including retroviruses, adenoviruses, and the Sinbis virus have been developed over the last decade for foreign gene expression in mammalian cells. This chapter will discuss the use of a replication-incompetent retroviral vector in cell culture to express an exogenous protein.

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https://doi.org/10.1007/978-1-4302-4570-4n mast cells and neutrophils, the major subclasses of PC are 1-acyl- and 1-alkyl-linked, whereas 1-acyl- and 1-alk-1-enyl-linked subclasses constitute the bulk of arachidonate in PE (.–.). PI is composed mainly of 1-acyl-linked species in these cells.

從屬

https://doi.org/10.1007/978-1-4842-3688-8wn concentration of a labeled eicosanoid with an unknown quantity of the same unlabeled eicosanoid. The antibody used has been generated against the specific eicosanoid to be measured. EIA is similar to RIA as an assay except that instead of using a radiolabeled compound, the competing antigen is labeled with a specific enzyme.

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lanugo

Enzyme immunoassays (EIA) of Eicosanoids,wn concentration of a labeled eicosanoid with an unknown quantity of the same unlabeled eicosanoid. The antibody used has been generated against the specific eicosanoid to be measured. EIA is similar to RIA as an assay except that instead of using a radiolabeled compound, the competing antigen is labeled with a specific enzyme.

TAP

Transcellular Regulation of Eicosanoid Biosynthesis,s, lipid mediators, nitric oxide, and chemoattractants. It thus has become apparent that transcellular eicosanoid biosynthesis during these interactions is an important means to amplify production of and/or results in the formation of novel mediators that neither cell type can generate alone (.–.).

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Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts,es by appropriate stimuli. This chapter describes methods used for the detection of PGS2 expression by western and northern analyses in murine Swiss 3T3 fibroblasts. These methods have also been used successfully to detect PGS2 expression in a number of other murine cell lines (.).