Titlebook: Effector-Triggered Immunity; Methods and Protocol Thomas A. Kufer,Maria Kaparakis-Liaskos Book 2022 The Editor(s) (if applicable) and The A

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Lambda Red Recombineering in ,d by an incomplete understanding of the molecular mechanisms by which . spp. causes disease and difficulties in manipulating . spp. genomes. While homologous recombination protocols for the construction of precise gene deletions exist, construction of mutants in . has not become commonplace. We desc

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Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of erial effector molecules, and can trigger a range of responses in the host. The study of BMV production, composition, and functions can give insights into their roles in mediating bacterial survival, pathogenesis, and disease. Furthermore, BMVs can be harnessed to develop cutting-edge nano-therapeut

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Host-Induced Gene Silencing Using BPMV on Soybean to Study Genes in the Soybean Rust Fungus ,or silence the respective gene. Since genetic transformation is not yet possible for rust fungi, silencing the gene is the only option. Posttranscriptional gene silencing uses RNAi. RNAi in plant pathogens can be accomplished by introducing dsRNA either by direct application of in vitro synthesized

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