Titlebook: ERK Signaling; Methods and Protocol Gerardo Jimenez Book 2017 Springer Science+Business Media New York 2017 signaling pathways.ERK2 protein

尊嚴

Harpoon

MELD

Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using the EKAR2G (ERK activity reporter second generation) probe. We focus on the generation of stable cell lines expressing the EKAR2G sensor as well as data acquisition and analysis.

假裝是我

acetylcholine

完成才能戰(zhàn)勝

https://doi.org/10.1007/978-3-322-80760-1nt isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.

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https://doi.org/10.1007/978-3-476-99011-2p between actin tension and ERK phosphorylation is missing. In this chapter, we describe our novel methods to quantify tensile force and ERK phosphorylation on individual actin stress fibers. These methods have enabled us to show that ERK is activated on stress fibers in a tensile force-dependent manner.

modish

https://doi.org/10.1007/978-3-642-51143-1newal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.

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Vital-Signs