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Titlebook: Dynamic Morphology of Leukemia Cells; A Comparative Study Heidi Felix,Gisela Haemmerli,Peter Str?uli Book 1978 Springer-Verlag Berlin · He

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書目名稱Dynamic Morphology of Leukemia Cells
副標(biāo)題A Comparative Study
編輯Heidi Felix,Gisela Haemmerli,Peter Str?uli
視頻videohttp://file.papertrans.cn/284/283678/283678.mp4
圖書封面Titlebook: Dynamic Morphology of Leukemia Cells; A Comparative Study  Heidi Felix,Gisela Haemmerli,Peter Str?uli Book 1978 Springer-Verlag Berlin · He
描述Dynamic Morphology is the attempt to correlate surface architec- ture and shape of fixed cells, as visualized by scanning electron microscopy (SEM), with the behavior of living cells, recorded by microcinematography (MCM). If SEM and MCM are used concurrently for the analysis of cell populations, a dynamic inter- pretation of SEM photographs is only valid if the experimental conditions are identical for the two techniques. This is achieved by allowing the cells to settle on a glass surface where they remain long enough to perform their various activities under conditions identical for both techniques (for technical details see Methodology). The analysis of a population necessitates the study of a large number of cells. This prerequisite is met by operating the scanning electron micro- scope at low levels of magnification, and by using culture chambers for cinematography. It can be argued that the examina- tion of attached cells excludes a complete SEM survey of a population, as cells not adhering from the outset or becoming detached during the different preparatory steps are lost. For this, cinematography proved to be a reliable control: All cell types recognized in time-lapse film
出版日期Book 1978
關(guān)鍵詞Cells; Morphology; behavior; cell; electron microscopy; leukemia; methodology; microscopy; population
版次1
doihttps://doi.org/10.1007/978-3-642-66794-7
isbn_softcover978-3-642-66796-1
isbn_ebook978-3-642-66794-7
copyrightSpringer-Verlag Berlin · Heidelberg 1978
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be argued that the examina- tion of attached cells excludes a complete SEM survey of a population, as cells not adhering from the outset or becoming detached during the different preparatory steps are lost. For this, cinematography proved to be a reliable control: All cell types recognized in time-lapse film978-3-642-66796-1978-3-642-66794-7
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978-3-642-66796-1Springer-Verlag Berlin · Heidelberg 1978
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Die Funktionsbereiche operativer Einheiten,Samples of heparinized venous blood from 19 untreated leukemia patients were obtained from the Department of Internal Medicine and the Children’s Hospital, both from the University of Zürich. From two adult patients an additional blood specimen was studied during the period of treatment (for technical details see Methodology).
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A,Rat leukemia cells were harvested from the peritoneal cavity, suspended in medium (BME, supplemented with 20% fetal calf serum, 0.03% glutamine and Hepes in a 0.01 . concentration), and immediately introduced into assembled culture chambers [Rose, 1954; Dvorak and Stotler, 1971]. The chambers were then transfered to 37° C.
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B,The hematologic data were kindly provided by the Department of Internal Medicine and the Children’s Hospital, both from the University of Zürich.
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發(fā)表于 2025-3-23 06:41:29 | 只看該作者
MaterialSamples of heparinized venous blood from 19 untreated leukemia patients were obtained from the Department of Internal Medicine and the Children’s Hospital, both from the University of Zürich. From two adult patients an additional blood specimen was studied during the period of treatment (for technical details see Methodology).
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