Titlebook: Genome Editing in Biomedical Sciences; Geraldo A. Passos Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Genome Editing in Biomedical Sciences978-3-031-33325-5Series ISSN 0065-2598 Series E-ISSN 2214-8019

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Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line,he oncogenesis of thyroid cancer is related to genetic alterations in MAPK signaling that induce proliferation and modulate noncoding genes, such as microRNAs and long noncoding RNAs. In this context, CRISPR/Cas9 emerges as a potential tool to modify gene sequence and modulate gene expression in thy

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CRISPR Libraries and Whole-Genome Screening to Identify Essential Factors for Viral Infections, activation, or editing. The advances in this technique make possible the development of CRISPR libraries which consist of a set of sgRNAs to cause perturbations in several genes in the same cell population. The use of libraries raised the CRISPR-Cas9 technique to a genomic scale and provides a powe