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Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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發(fā)表于 2025-3-21 18:10:54 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Drosophila
副標(biāo)題Methods and Protocol
編輯Christian Dahmann
視頻videohttp://file.papertrans.cn/283/282971/282971.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive
描述This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful .Methods in Molecular Biology .series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.?Authoritative and cutting-edge,?.Drosophila: Methods and Protocols, Third Edition.?serves as a useful and practical guide to new researchers and experts using Drosophila as a model system..Chapter 7 is available open access under a Creative
出版日期Book 2022Latest edition
關(guān)鍵詞FlyBase; Gene Expression; CRISPR-Cas9; Optogenetic; Electrophysical recordings; Drosophila melanogaster
版次3
doihttps://doi.org/10.1007/978-1-0716-2541-5
isbn_softcover978-1-0716-2543-9
isbn_ebook978-1-0716-2541-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

書目名稱Drosophila影響因子(影響力)




書目名稱Drosophila影響因子(影響力)學(xué)科排名




書目名稱Drosophila網(wǎng)絡(luò)公開(kāi)度




書目名稱Drosophila網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書目名稱Drosophila被引頻次




書目名稱Drosophila被引頻次學(xué)科排名




書目名稱Drosophila年度引用




書目名稱Drosophila年度引用學(xué)科排名




書目名稱Drosophila讀者反饋




書目名稱Drosophila讀者反饋學(xué)科排名




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發(fā)表于 2025-3-22 00:02:28 | 只看該作者
Dark and Light Adaptation: Psychophysics,unction in . with a particular focus on the large-scale resources that enable systematic analysis. Application of these methods will accelerate the discovery of ways that miRNAs are embedded into genetic networks that control basic features of metazoan cells.
板凳
發(fā)表于 2025-3-22 04:19:48 | 只看該作者
Models for Vector-Borne Parasitic Diseasesd from indirect in vivo techniques. Thus, this method provides an improved way of studying dynamic cellular processes and tissue movements during imaginal disc development. I first describe the preparation of the growth?medium and the dissection, and then I include a protocol for mounting and live imaging of the explants.
地板
發(fā)表于 2025-3-22 06:30:57 | 只看該作者
The Q-system: A Versatile Repressible Binary Expression System,plication of the Q-system for transgenic labeling, the modular nature of QF that allows chimeric or split transcriptional activators to be developed, its temporal control by quinic acid, new methods to generate QF2 reagents, intersectional expression labeling, and its recent adoption into many emerging experimental species.
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發(fā)表于 2025-3-22 11:15:25 | 只看該作者
Resources and Methods for the Analysis of MicroRNA Function in ,unction in . with a particular focus on the large-scale resources that enable systematic analysis. Application of these methods will accelerate the discovery of ways that miRNAs are embedded into genetic networks that control basic features of metazoan cells.
6#
發(fā)表于 2025-3-22 13:39:21 | 只看該作者
Cultivation and Live Imaging of , Imaginal Discs,d from indirect in vivo techniques. Thus, this method provides an improved way of studying dynamic cellular processes and tissue movements during imaginal disc development. I first describe the preparation of the growth?medium and the dissection, and then I include a protocol for mounting and live imaging of the explants.
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發(fā)表于 2025-3-22 21:00:37 | 只看該作者
1064-3745 ation advice from the expertsThis third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single
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發(fā)表于 2025-3-22 22:33:05 | 只看該作者
https://doi.org/10.1007/978-3-642-88653-9discovery. This chapter aims to give users new to the database an overview of the layout and types of data available, as well as introducing some tools with which to access the data. More experienced users will find useful information about recent improvements and descriptions to enable more efficient navigation of the database.
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發(fā)表于 2025-3-23 02:27:33 | 只看該作者
Affine und projektive Geometrierrors than traditional methods. Initially described in mammalian cells, prime editing is functional in . somatic and germ cells. Here, we outline steps to design, generate, and express prime editing components in transgenic flies. Furthermore, we highlight a crossing scheme to produce edited fly stocks in less than 3?months.
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發(fā)表于 2025-3-23 05:59:28 | 只看該作者
Rudolf Lapierre,Gerd Steierwald functional analyses. In this chapter, we describe the preparation of . tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing–freeze substitution (HPF–FS) to preserve cellular structures.
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