Titlebook: Disease Gene Identification; Methods and Protocol Johanna K.‘DiStefano Book 2018Latest edition Springer Science+Business Media, LLC 2018 bi

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The Emerging Role of Long Noncoding RNAs in Human Diseaseanscriptionally silent, but recent large-scale investigations have instead revealed a rich array of functionally significant elements, including non-protein-coding transcripts, within the noncoding regions of the human genome. Long noncoding RNAs (lncRNAs), a class of noncoding transcripts with leng

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Whole Exome Library Construction for Next Generation Sequencingns of interest. As the coding portion of the genome encompasses only 1–2% of the entire genome, this approach represents a more cost-effective strategy to detect DNA alterations that may alter protein function, compared to whole genome sequencing. Although the research community has and is currently

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Using Fluidigm C1 to Generate Single-Cell Full-Length cDNA Libraries for mRNA Sequencingiation, disease progression, and gene regulation in a multitude of research areas. The generation of high-quality cDNA, an important step in the experimental workflow when generating sequence-ready libraries, is critical to maximizing data quality. Here we describe a strategy that uses a microfluidi

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MiSeq: A Next Generation Sequencing Platform for Genomic Analysisd sequencing solutions. The MiSeq instrument is one of the smallest benchtop sequencers that can perform onboard cluster generation, amplification, genomic DNA sequencing, and data analysis, including base calling, alignment and variant calling, in a single run. It performs both single- and paired-e

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Methods for CpG Methylation Array Profiling Via Bisulfite Conversiond epigenetic events such X inactivation, cellular differentiation and proliferation, and embryonic development. The most conserved epigenetic modification in plants, animals, and fungi is 5-methylcytosine (5mC), which has been well characterized across a diverse range of species. Many technologies h

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miRNA Quantification Method Using Quantitative Polymerase Chain Reaction in Conjunction with ,, Methind to the 3′untranslated region (3′UTR) of target mRNAs bearing complementary sequences, and target more than half of all protein-coding genes in the human genome. The dysregulation of miRNA expression and activity has been linked with numerous diseases, including cancer, cardiovascular diseases, n