Titlebook: Directed Evolution Library Creation; Methods and Protocol Frances H. Arnold,George Georgiou Book 2003 Humana Press 2003

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書目名稱Directed Evolution Library Creation影響因子(影響力)




書目名稱Directed Evolution Library Creation影響因子(影響力)學(xué)科排名




書目名稱Directed Evolution Library Creation網(wǎng)絡(luò)公開度




書目名稱Directed Evolution Library Creation網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Directed Evolution Library Creation被引頻次




書目名稱Directed Evolution Library Creation被引頻次學(xué)科排名




書目名稱Directed Evolution Library Creation年度引用




書目名稱Directed Evolution Library Creation年度引用學(xué)科排名




書目名稱Directed Evolution Library Creation讀者反饋




書目名稱Directed Evolution Library Creation讀者反饋學(xué)科排名

fetter

Guglielmo Chiodi,Leonardo Dittaversity is generated entirely in vitro, and the protocol, involving two rounds of PCR with no gel purifications, can be accomplished easily in less than one day, followed by cloning as one would for a traditional PCR product.

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Construction of Designed Protein Libraries Using Gene Assembly Mutagenesisversity is generated entirely in vitro, and the protocol, involving two rounds of PCR with no gel purifications, can be accomplished easily in less than one day, followed by cloning as one would for a traditional PCR product.

Cardioversion

平靜生活

Saturation Mutagenesisms, and study structure-function relationships. With saturation mutagenesis, it is possible to create a library of mutants containing all possible mutations at one or more pre-determined target positions in a gene sequence.

平靜生活

DNA Shuffling annealing, and extension by a polymerase (..). Recombination occurs when fragments from different parents anneal at a region of high sequence identity. Following this reassembly reaction, PCR amplification with primers is used to generate full-length chimeras suitable for cloning into an expression vector.

確定方向

In Vitro DNA Recombination by Random Priminggical systems (.,.). As compared to random mutagenesis, recombination may be advantageous in combining beneficial mutations that have arisen independently and may be synergistic, while simultaneously removing deleterious mutations.

松軟

Preparation of SCRATCHY Hybrid Protein Librariese identity between the original sequences (.). Such multi-crossover hybrids can be of interest to the studies of fundamental questions of protein evolution and folding, as well as to the tailoring of enzymes for therapeutic and industrial applications.

Allodynia