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Titlebook: Directed Evolution; Methods and Protocol Andrew Currin,Neil Swainston Book 2022 Springer Science+Business Media, LLC, part of Springer Natu

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書目名稱Directed Evolution
副標(biāo)題Methods and Protocol
編輯Andrew Currin,Neil Swainston
視頻videohttp://file.papertrans.cn/281/280662/280662.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Directed Evolution; Methods and Protocol Andrew Currin,Neil Swainston Book 2022 Springer Science+Business Media, LLC, part of Springer Natu
描述.This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Cutting-edge and comprehensive, .Directed Evolution: Methods and Protocols. is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows..
出版日期Book 2022
關(guān)鍵詞SelFi; computationally guided protein stapling; De novo protein design; gene synthesis; mutagenesis
版次1
doihttps://doi.org/10.1007/978-1-0716-2152-3
isbn_softcover978-1-0716-2154-7
isbn_ebook978-1-0716-2152-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
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書目名稱Directed Evolution影響因子(影響力)




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書目名稱Directed Evolution網(wǎng)絡(luò)公開度學(xué)科排名




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Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries,hods for multiple site-saturation mutagenesis, such as Darwin Assembly, can accelerate the sampling of relevant sequence space and the identification of variants with desired functionalities. Here, we present the automation of the Darwin Assembly method, using a Gilson PIPETMAX? liquid handling plat
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SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Varprovements such as . thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein’s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address
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GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes,or stability. In order to achieve these desired improvements, it is often beneficial to subject the entirety of the protein to mutagenesis. However, the creation of such libraries by targeted methods (i.e. site-directed mutagenesis) can be a laborious and costly task. Here we outline the GeneORator
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Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs,ction of strong prokaryotic promoters that can be recognized by designated multiple sigma factors by interlocking their cognate binding motifs on DNA strands. Strong and stress responsive promoters for . and . have been created following the presented protocol. Customized promoters could be easily d
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