Titlebook: Directed Enzyme Evolution; Screening and Select Frances H. Arnold,George Georgiou Book 2003 Humana Press 2003

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High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450 are easily modified for high-throughput in microtiter plates. This method is useful in quickly assaying a library of cytochrome P450s for folded and possibly functional proteins while eliminating misfolded or low expressing variants. It can also be used to rapidly determine the P450 concentration o

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Müfit Bahadir,Harun Parlar,Michael Spitelleren used in conjunction with directed molecular evolution. Our lab has used this approach to analyze the function of enzymes involved in DNA metabolism, to study the mutability of protein domains, and to generate mutant proteins possessing properties different from those selected by natural evolution

思考

Müfit Bahadir,Harun Parlar,Michael Spitellernditions is restored by small amounts of DNA polymerase activity. Even mutants with greatly reduced (1–10% of wild-type) catalytic activity or distantly-related polymerases of bacterial, eukaryotic, or viral origin effectively complement JS200 cells. The versatility of this complementation system ma

CREST

Müfit Bahadir,Harun Parlar,Michael Spitellerymes such as DNA polymerases, which carry out pivotal role during DNA replication, repair, and recombination, are poorly conserved amongst different families, but within a given family, all the members are highly conserved. These observations have profound implications and suggest that DNA polymeras

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https://doi.org/10.1007/978-3-642-56998-2r its application as an industrial enzyme. The reason for low solubility can lie in low conformational stability (.), in a high number of surface-exposed hydrophobic amino acids (.) or in certain structural features, such as membrane binding regions (.). By changing the amino acid sequence of these