Titlebook: Differential Display Methods and Protocols; Peng Liang,Arthur B. Pardee Book 19971st edition Humana Press 1997

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https://doi.org/10.1007/978-1-4939-7108-4tially expressed mRNAs (i.e., using plus/minus screening); but if the quest is to identify the majority of differentially expressed mRNA species, then a method that is not sensitive to mRNA abundance is required. To evaluate how well DD and SH can identify abundant as well as rare differentially exp

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Book 19971st editionmRNAs in a cell or tissue, in particular those with altered expression resulting from diff- ences in transcription or mRNA degradation. In contrast to conventional te- niques, DD can be used to compare mRNA expressions in many samples created under multiple experimental conditions. DD was conceived

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Differential Displayon and characterization of differentially expressed genes becomes one of the first steps toward the understanding of these important biological questions. Differential display (.) and a related RAP-PCR method (.) were developed to more efficiently identify and isolate these genes.

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Direct Sequencing of Differential Display PCR Productsnchored primers, T.MN (M = G, A or C; N = G, A, T or C); and (2) PCR amplification of cDNA fragments using arbitrary 10-mer primers (upstream) and anchored downstream primers. One critical feature of this technique is to display most of the mRNA population on a sequencing gel after PCR.

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A Direct-Sequencing-Based Strategy for Identifying and Cloning cDNAs from Differential Display Gelsther from a variety of sources in a revised order. Methods for the DD reverse transcription-polymerase chain reactions (RT-PCR) and electrophoresis conditions, for which we have used published methods (.) and the GenomyxLR DNA sequencer (.. and ref. .), are not covered here.

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RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Displayreakthrough in molecular biology, because they circumvented some severe limitations of traditional methods, such as differential screening and subtractive hybridization, during the analysis of differential gene expression.