| 書目名稱 | Differential Display Methods and Protocols | | 編輯 | Peng Liang,Arthur B. Pardee | | 視頻video | http://file.papertrans.cn/279/278659/278659.mp4 | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Of the estimated 80,000 individual genes encoded by the human genome, approximately 10-20% are expressed by the average cell. This subset is a major determinant of a cell‘s properties. In addition to the control of cellular phenotype and the normal physiological processes of an organism, an alt- ation in gene expression (either induction or repression) underlies the etiology of numerous, diverse pathological processes. Therefore understanding the mechanisms of these normal and pathological processes requires identifi- tion, isolation, and characterization of differentially expressed genes. This requirement has led to the development of a variety of techniques capable of identifying small quantities of proteins or mRNAs that are key to a multitude of diverse pathological processes. Differential display (DD) is an emerging "fingerprinting" technology that facilitates the identification of mRNAs in a cell or tissue, in particular those with altered expression resulting from diff- ences in transcription or mRNA degradation. In contrast to conventional te- niques, DD can be used to compare mRNA expressions in many samples created under multiple experimental conditions. DD was conceived | | 出版日期 | Book 19971st edition | | 版次 | 1 | | doi | https://doi.org/10.1385/0896034895 | | isbn_ebook | 978-1-59259-569-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 1997 |
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