Titlebook: Difference Gel Electrophoresis; Methods and Protocol Kay Ohlendieck Book 2023Latest edition The Editor(s) (if applicable) and The Author(s)

膽小懦夫

倒轉(zhuǎn)

Minatory

https://doi.org/10.1007/978-94-009-7526-2 enzymes, and deubiquitinating proteases. Using fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) to detect and quantitate cellular proteins associated with the ubiquitination process will facilitate the evaluation of this post-translational modification associated with the pathophysiological phenotype.

思想

Quantification of Circulating Proteinser technology utilizes hexapeptide bead library with huge diversity to bind and enrich low-abundance proteins but at the same time suppresses the concentration of high-abundance proteins in subsequent analysis.

考博

Turnover and Distribution of Plasma Proteinsn detail. A standardized proteomic workflow is described, involving sample preparation, protein extraction, differential fluorescence labeling using a 3-CyDye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, 2D-DIGE image analysis, protein digestion, and mass spectrometry.

Little

Quadrilingual Economics Dictionaryantly, saliva represents a body fluid that is continuously available for diagnostic and prognostic assessments. This chapter gives an overview of saliva proteomics, including a discussion of the usefulness of both liquid chromatography and two-dimensional gel electrophoresis for efficient protein separation in saliva proteomics.