Titlebook: Diabetic Nephropathy; Methods and Protocol Luigi Gnudi,David A. Long Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Diabetic Nephropathy: An Overview. The classical presentation of DN is characterized by hyperfiltration and albuminuria in the early phases which is then followed by a progressive renal function decline. The presentation of diabetic kidney disease (DKD) can vary especially in patients with T2DM where concomitant presence of other g

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Modeling Podocyte Biology Using , Nephrocyteses known as slit diaphragms that contribute to the glomerular filtration barrier. The disruption of podocyte biology or slit diaphragm formation in humans is a precursor to albuminuria, renal failure, and cardiovascular morbidity. Due to genetic and functional similarities, the nephrocytes of . are

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A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephrosdevelop translatable in vivo models to study genetic changes. This allows for functional validation of pathogenic mutations and establishes a system to understand the etiology of disease. Due to the ease of genetic manipulation and rapid . development, the zebrafish has become a valuable resource to

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Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic R progressive disease or test novel therapeutics. In this chapter we describe in detail the experimental procedures required to conduct the . rodent model, in which hyperglycemia and renin-dependent hypertension synergize to promote moderate proteinuria, renal fibrosis, and induction of many of the t

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Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopymergence of numerous tissue clearing strategies, and advancements in confocal and two-photon microscopy, enabling the study of intact organs and systems down to cellular and sub-cellular resolution. In this chapter, we describe a wholemount immunofluorescence technique for labeling structures in ren

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