Titlebook: Developmental Biology of the Sea Urchin and Other Marine Invertebrates; Methods and Protocol David J. Carroll,Stephen A. Stricker Book 2014

interpose

性滿足

禁止

真實的你

憤怒歷史

Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian ,,ortical structures like the CAB in ascidians, etc.) combined with targeted gene knockdown during embryonic development is a powerful technique to understand the mechanisms of embryonic development. The eggs and embryos of the primitive marine chordate . are an excellent model system for combining li

支形吊燈

moribund

Biotinylation of Oocyte Cell Surface Proteins of the Starfish , ,rom the cell surface to relevant intracellular compartments. To identify starfish oocyte and egg cell surface proteins, a biotinylation method was adapted from prior protocols using B cells, leukocytes, mouse oocytes, and sea urchin eggs (Cole et al. Mol Immunol 24:699–705, 1987; Flaherty and Swann

Canary

喊叫

閃光你我

Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte Maturation in Marine Nar extracts. This chapter describes a traditional, film-based immunoblotting method for monitoring the phosphorylation status of proteins in marine nemertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular p