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Titlebook: Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis; Bin Zhuang Book 2018 Springer Nature Singa

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發(fā)表于 2025-3-21 18:09:01 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis
編輯Bin Zhuang
視頻videohttp://file.papertrans.cn/271/270012/270012.mp4
概述Nominated by Tsinghua University as an outstanding Ph.D. thesis.Develops a disposable DNA extraction system and PCR microchip, as well as a compact control instrument for genetic testing of hereditary
叢書名稱Springer Theses
圖書封面Titlebook: Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis;  Bin Zhuang Book 2018 Springer Nature Singa
描述.This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice..
出版日期Book 2018
關(guān)鍵詞Integrated Module; Capillary Electrophoresis; Congenital Hearing Loss; Composite Chip; Micro-fluidic Tec
版次1
doihttps://doi.org/10.1007/978-981-10-4753-4
isbn_softcover978-981-13-5220-1
isbn_ebook978-981-10-4753-4Series ISSN 2190-5053 Series E-ISSN 2190-5061
issn_series 2190-5053
copyrightSpringer Nature Singapore Pte Ltd. 2018
The information of publication is updating

書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis影響因子(影響力)




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis影響因子(影響力)學(xué)科排名




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis網(wǎng)絡(luò)公開度




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis被引頻次




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis被引頻次學(xué)科排名




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis年度引用




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis年度引用學(xué)科排名




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis讀者反饋




書目名稱Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis讀者反饋學(xué)科排名




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Grundriss der pharmaceutischen Chemied. Different kinds of approaches have their own advantages and application occasions. Microfluidic is a newly developed technology that aims at precisely manipulating fluidic of micro-scale. The microfluidic made it possible to integrate tradition genetic analysis process onto one single microchip f
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https://doi.org/10.1007/978-3-642-91504-8te the chip, was designed and fabricated. On-chip extraction was performed by twining long-chain nucleic acid molecules onto the filter paper. And the thermal cycles were then applied directly to the filter paper with DNA twined on for PCR amplification. To precisely manipulate on-chip fluidic and f
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發(fā)表于 2025-3-22 13:40:16 | 只看該作者
Grundriss der pharmaceutischen Chemieated genetic analysis. The chips and the instrument were also upgraded correspondingly. The upgraded integrated chip is a compound material one that uses plastic chip for sample preparation and glass chip for capillary electrophoresis detection. The cheap plastic chip is disposable while the glass c
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發(fā)表于 2025-3-22 17:25:47 | 只看該作者
Grundriss der pharmaceutischen Chemieapter and offer some prospects for future works. We hope the system would be able to integrate more modules, deal with more various kinds of samples and be applied in more areas. We would be quite happy if our research works do help more and more people.
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