Titlebook: Deubiquitinases; Methods and Protocol Julie Maupin-Furlow,Mariola J. Edelmann Book 2023 The Editor(s) (if applicable) and The Author(s), un

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Navdeep Agrawal,Jagabandhu Dixit analysis of DUB activity. DUB activity can be measured in purified enzyme reactions, in cell lysates, or in intact cells depending upon the choice of the fluorometric probe. This chapter describes protocols and potential analysis tools to investigate DUB activity in these three scenarios.

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Lars Kulik,Egemen Tanin,Muhammad Umer in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in . to overcome these challenges and identify DUB substrates in cells.

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Design and Synthesis of Ubiquitin-Based Chemical Tools with Unnatural Amino Acids for Selective Dettorial substrate library (HyCoSuL) approach that can be introduced in the C-terminal motif of Ub. Furthermore, we describe the synthesis protocol of Ub-based probes and substrates containing unnatural amino acids and the application of Ub-based probes to detect DUBs in cell lysates.

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