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Titlebook: Design and Delivery of SiRNA Therapeutics; Henrik J. Ditzel,Martina Tuttolomondo,Sakari Kaupp Book 2021 Springer Science+Business Media, L

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書目名稱Design and Delivery of SiRNA Therapeutics
編輯Henrik J. Ditzel,Martina Tuttolomondo,Sakari Kaupp
視頻videohttp://file.papertrans.cn/269/268543/268543.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Design and Delivery of SiRNA Therapeutics;  Henrik J. Ditzel,Martina Tuttolomondo,Sakari Kaupp Book 2021 Springer Science+Business Media, L
描述.This volume details protocols on rationale design of therapeutic siRNA molecules and its encapsulation with smart vehicles to overcome the barriers to an effective administration?.in vivo.. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,?.Design and Delivery of SiRNA Therapeutics .aims to ensure successful results in the further study of this vital field..
出版日期Book 2021
關(guān)鍵詞RNA interference; Therapeutic strategy; Regulatory processes; Conjugation strategies; Non-coding small R
版次1
doihttps://doi.org/10.1007/978-1-0716-1298-9
isbn_softcover978-1-0716-1300-9
isbn_ebook978-1-0716-1298-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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,Engineering of Solid Dosage Forms of siRNA-Loaded Lipidoid–Polymer Hybrid Nanoparticles Using a Qua-glycolic acid) to effectively deliver siRNA directed against tumor necrosis factor alpha (TNF-α) intracellularly to macrophages. L. is a novel lipid-like material consisting of a tetraamine backbone linked to five C. alkyl chains. Here, we describe a systematic quality-by-design (QbD) approach incl
地板
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Cell-Penetrating Peptides Delivering siRNAs: An Overview,thesis, use, and versatility. Recent progress with siRNA delivered by CPPs seems to focus on targeted delivery to reduce side effects and amount of drugs used, and it appears to be among the most promising use for CPPs in future clinical applications.
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siRNA Vehicles for High Endosomal Escapability, a highly required characteristic for the induction of a high knockdown effect. Here, we describe the step-by-step procedure for the evaluation of high endosomal escapability. The vector that has pH-responsive characteristics at around pH?=?6.2–6.5 is important for the high endosomal escape.
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siRNA Design and Delivery Based on Carbon Nanotubes,he past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.
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