Titlebook: Dendritic Cell Protocols; Elodie Segura,Nobuyuki Onai Book 2016Latest edition Springer Science+Business Media New York 2016 culture human

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Katja Schechtner,Melinda Hansont dendritic cells are heterogeneous and comprise several subsets that can be distinguished by their surface phenotype, ontogeny, and molecular signature. This review gives an overview of mouse and human dendritic cell subsets and their defining features and summarizes the current knowledge of dendri

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Does Your Idea Have Disruptive Potential?Most of the information gained on human DC biology was derived from studies conducted with DCs generated in vitro from peripheral blood CD14. monocytes (MoDCs) or from CD34. hematopoietic progenitors. Recent advances in the field revealed that these types of in vitro-derived DCs strikingly differ fr

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Modularisierung und Plattformenr understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.

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Christoph Fuchs,Franziska J. Golenhofenls from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small a

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Christoph Fuchs,Franziska J. Golenhofen anatomical location, phenotype and function. In human, the two most accessible tissues to study leukocytes are peripheral blood and skin. DCs are rare in human peripheral blood (<1?% of mononuclear cells) and have a less mature phenotype than their tissue counterparts (MacDonald et al., Blood. 100:

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