Titlebook: Decoding the Antibody Repertoire; High Throughput Sequ Brandon DeKosky Book 2017 Springer International Publishing AG 2017 B Cell.Single-ce

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Bennett T. Amaechi BDS, MS, PhDs work was undertaken specifically to address a critical deficiency in currently available high throughput antibody sequencing techniques, namely that the pairing information of heavy and light chains is irreversibly lost during traditional high-throughput sequencing. We first reported a microarray-

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Brandon DeKoskyNominated as an outstanding Ph.D thesis by the University of Texas at Austin.Outlines the development of the very first technology for high-throughput analysis of paired heavy and light chain antibody

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https://doi.org/10.1007/978-3-319-60961-4f VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [.]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size of the human antibody repertoire) are identified via scRT-PCR [.].

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https://doi.org/10.1007/978-3-319-60961-4The last 30?years of the biotechnology revolution have to a great extent been fueled by the discovery and application of monoclonal antibodies for research and therapeutic purposes.