Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot

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Eugene Valkov,Aaron C. GoldstrohmIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/263953.jpg

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Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.

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Transcriptome-Wide Analysis of mRNA Adenylation Status in Yeast Using Nanopore Sequencing,ics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.

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Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.

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