Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot

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Sequencing of Transcriptome-Wide Poly(A) Tails by PAIso-seq,inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.

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Differential Poly(A) Tail Length Analysis Using Nanopore Sequencing,senger RNAs. As a result, an accurate measurement of poly(A) tail length changes is important for understanding its regulatory function in different cellular contexts. Here, we outline a method for using nanopore sequencing and linear mixed models to analyze differences in poly(A) tail length across conditions.

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Gonzalo L. Villarreal,Verónica Artolas, with or without poly(A) tails. Finally, we also demonstrate the adaptability of these assays to test purified protein components in our cell-free deadenylation assay. In our experience, these methods are well suited for the initial assessment of the effects of RBPs on the deadenylation of mRNAs.

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