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Titlebook: DNA and RNA Profiling in Human Blood; Methods and Protocol Peter Bugert Book 2009 Humana Press 2009 Blood cell antigens.Disease markers.Gen

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書(shū)目名稱DNA and RNA Profiling in Human Blood
副標(biāo)題Methods and Protocol
編輯Peter Bugert
視頻videohttp://file.papertrans.cn/261/260219/260219.mp4
概述Details numerous techniques to perform blood group genotyping, covering prenatal blood grouping from maternal plasma to multiplex HLA genotyping.Presents not only established but also cutting-edge dev
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: DNA and RNA Profiling in Human Blood; Methods and Protocol Peter Bugert Book 2009 Humana Press 2009 Blood cell antigens.Disease markers.Gen
描述.Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In .DNA and RNA Profiling in Human Blood: Methods and Protocols., leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful .Methods in Molecular Biology.? series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .DNA and RNA Profiling in Human Blood: Methods and Protocols. is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and
出版日期Book 2009
關(guān)鍵詞Blood cell antigens; Disease markers; Genotyping; High-throughput multiplex approaches; Megakaryocytes; P
版次1
doihttps://doi.org/10.1007/978-1-59745-553-4
isbn_softcover978-1-62703-913-0
isbn_ebook978-1-59745-553-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Single Base Extension in Multiplex Blood Group Genotypingrnative strategy to phenotyping blood is to assay genomic DNA for the associated single nucleotide polymorphisms (SNPs). A multiplex PCR coupled with a single base oligonucleotide extension assay using genomic DNA can identify SNPs related to D, C/c, E/e, S/s, K/k, Kp., Fy., Fy0 (–33 promoter silenc
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Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevances, cancer, or infections and genetically determined susceptibility to danger signals may influence the development of inflammatory diseases. Members of the ‘toll-like receptor’ (TLR) family are pivotal molecules in the activation of the innate immune system and specifically recognize structurally co
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High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Appriation involves differences in single DNA nucleotides, and is thus termed single nucleotide polymorphism (SNP). The need for improvement in throughput and reliability of traditional techniques makes it necessary to develop new technologies. Thus the past few years have witnessed an extraordinary su
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Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeadsn genetic variation and disease. Discoveries made by such whole-genome association studies often spur further interest in surveying more focused subsets of SNPs for validation or research purposes. Here we describe a new SNP genotyping platform that is flexible in assay content and multiplexing (up
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